รองศาสตราจารย์ ดร.สมชัย พรบันลือลาภ

Assoc.Prof. Somchai Pornbanlualap

  • Education Background

    1995 Ph.D. Biochemistry, School of Medicine, Temple University, Philadelphia, PA, USA. 1995-98 Postdoctoral Molecular biology, University of California at Berkeley, Berkeley, CA, USA
    1990 M.S. Biochemistry, School of Medicine, Temple University, Philadelphia, PA, USA
    1987 B.A. Biochemistry, School of Medicine,Temple University, Philadelphia, PA, USA

  • Area of Expertise

    Nucleoside/nucleotide and nucleic acids biochemistry
    Catalytic and kinetic mechanisms of enzymes
    Nucleoside analogs as biological probes
    Biosynthesis of nucleoside antibiotics

  • Research Interests:

    One of the projects in our laboratory is elucidation of the biosynthesis of nucleoside analogs such as 2’-deoxycoformyin, coformycin, and arabinofuranosyladenine (ara-A) in Streptomcyes. To clone the antibiotic gene cluster from Streptomyces, various approaches including functional complementation, PCR, and inverse PCR have been exploited. A novel antibiotic resistant gene, the adenosine deaminase that is not inhibited by the transition state analog 2’-deoxycoformycin, had been cloned and its sequence determined. The kinetic and catalytic properties of this enzyme have been under investigation.
    The second project involves enzymatic synthesis of nucleosides/nucleotides and their analogs. These analogs have been important biochemical tools for elucidation of the mechanisms of DNA replication, transcription, and translation, mechanism of enzymatic reaction, and subcellular organization. Several nucleoside antibiotics have been used in the treatment of various diseases. Furthermore, deoxyribonucleotide triphosphates (dNTP) have becoming indispensable tool used in polymerase chain reaction. To enzymatically synthesize nucleosides/nucleotides and their analogs, recombinant polyhistidine-tagged enzymes including adenylate kinase, CMP kinase, deoxyribonucleoside kinase, nucleoside diphosphate kinase, and pyruvate kinase had been cloned and overexpressed. These enzymes are immobilized onto nickel-agarose column in a semi-solid state for milligram- and gram-scale synthesis of the nucleotide.
    The third project involves cloning, expression, and rational design of fish growth hormone. Based on the available crystal structure of human growth hormone-receptor complex, more potent mutant growth hormones are being generated by site-specific mutagenesis and random mutagenesis. Effective methods of delivering fish growth hormone to fish have also been investigated in our laboratory.

Examples
of publications

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1. Howan P and Pornbanlualap S. (2003) Cloning and effective induction of Escherichia coli nucleoside diphosphate kinase by lactose. ScienceAsia, 29, 347-353. 

2. Vasakorn B, Suraphon V, Milne J. and Pornbanlualap S (2005) Effects of Mangosteen’s peel and rambutan seeds on toxicity, esterase and glutathione S-transferase in rice Weevil. Kasetsart J. 38, 84-89. 

3. Suhadolnik RJ, Pornbanlualap S, Wu J, Baker DC, and Hebbler AK (1989) Biosynthesis of 9-b-D-Arabinofuranosyladenine: Hydrogen exchange at C-2’ and oxygen exchange at C-3’ of adenosine. Arch. Biochem. Biophys. 270, 363-373.

4. Suhadolnik RJ, Pornbanlualap S, Baker DC, Tiwari KN, Hebbler AK and Baker DC (1989) Stereospecific 2’-amination and 2’-chlorination of adenosine by Actinomadura in the biosynthesis of 2’-amino-2’-deoxyadenosine and 2’-chloro-2’-doexycoformycin. Arch. Biochem. Biosphys. 270, 374-382.

5. Suhadolnik RJ, Hanvey JC, Pornbanlualap S, Wu J, Tiwari KN, and Hebbler AK, and Baker DC. (1989). Nucleoside & Nucleotides 8, 893-896.

ปีรายละเอียด
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(no date)สถาบันวิจัยแห่งมหาวิทยาลัยเกษตรศาสตร์
ปีรายละเอียด
  • นางสาวอัษฏะวรรณ สุนันทการกิจ (อุ๋ย) ( นิสิตปริญญาโท สาขาวิชาชีวเคมี)
  • นางสาวรวินท์ นราวงศานนท์ (เมย์) ( นิสิตปริญญาโท สาขาวิชาชีวเคมี)
  • นายชิโนไท พูน (แลน) (นิสิตปริญญาโท สาขาวิชาพันธุวิศวกรรม)